Description
Product name
Recombinant human CD47 protein (Active)See all CD47 proteins and peptidesBiological activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human CD172a / SIRP alpha Fc Chimera is coated at 1 µg/mL, ab174029 binds with apparent KD < 1="">
Purity
>95% SDS-PAGE.Endotoxin level
<>Expression system
HEK 293 cellsAccession
Q08722Protein length
Protein fragmentAnimal free
NoNature
RecombinantSpecies
HumanSequence
QLLFNKTKSV EFTFCNDTVV IPCFVTNMEA QNTTEVYVKW KFKGRDIYTF DGALNKSTVP TDFSSAKIEV SQLLKGDASL KMDKSDAVSH TGNYTCEVTE LTREGETIIE LKYRVVSWFS PPredicted molecular weight
16 kDa including tagsAmino acids
19 to 139Tags
His tag C-Terminus
Associated products
Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-6X His tag® antibody (ab9108)
Specifications
Our Abpromise guarantee covers the use ofab174029in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- Applications
ELISA
Functional Studies
SDS-PAGE
Form
LyophilizedAdditional notes
After reconstitution, store under sterile conditions for 1 month at 4°C - 8°C or 3 months at -20°C to -80°C.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40Constituents: PBS, 5% TrehaloseLyophilized from 0.22 µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
Alternative names
- Antigen identified by monoclonal antibody 1D8
- Antigenic surface determinant protein OA3
- CD 47
see allFunction
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.Tissue specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain.Cellular localization
Cell membrane.- Information by UniProt
