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商品描述
Cat. No.: 710 006DY1
Amount: 5 nmol
Price:355.00€
| Cat.No.710006DY1 | 5nmol mCLING labeled with DY®654 in 100µl PBS (lyophilized). For reconstitution add 100µl H2O, then aliquot and store at -80°C until use.Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye. |
| Applications | ICC:1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml)galleryIHC:1 : 25 up to 1 : 50 (1 - 2 nmol/ml) |
| Label | DY 654 |
| Remarks | Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4%paraformaldehyde (PFA) and 0.2% glutaraldehyde is strongly advised. For detailed protocols see ReveloNH&RizzoliSO,2016. |
| Data sheet | 710_006dy1.pdf |
