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商品描述
Cat. No.: 710 006AT1
Amount: 5 nmol
Price:355.00€
| Cat.No.710006AT1 | 5nmol mCLING labeled with ATTO®647N in 100µl PBS (lyophilized). For reconstitution add 100µl H2O, then aliquot and store at -80°C until use.Reconstitute immediately upon receipt! Avoid bright light when working with the probe to minimize photo bleeching of the fluorescent dye. |
| Applications | ICC:1 : 75 up to 1 : 250 (0.2 - 0.7 nmol/ml)galleryIHC:1 : 25 up to 1 : 50 (1 - 2 nmol/ml) |
| Label | ATTO 647N |
| Remarks | Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4%paraformaldehyde (PFA) and 0.2% glutaraldehyde is strongly advised. For detailed protocols see ReveloNH&RizzoliSO,2016. |
| Data sheet | 710_006at1.pdf |
References for mCLING-ATTO 647N-labeled - 710006AT1
A versatile and customizable low-cost 3D-printed open standard for microscopic imaging.DiederichB, LachmannR, CarlstedtS, MarsikovaB, WangH, UwurukundoX, MosigAS, HeintzmannRNature communications (2020) 111: 5979. 710006AT1 ICC; tested species: e. coli
Integrative characterization of the near-minimal bacterium Mesoplasma florum.MatteauD, LachanceJC, GrenierF, GauthierS, DaubenspeckJM, DybvigK, GarneauD, KnightTF, JacquesPÉ, RodrigueSMolecular systems biology (2020) 1612: e9844. 710006AT1 ICC
Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform That Coordinates Cytoskeletal and Signaling Components for Cytokinesis.SniderCE, ChandraM, McDonaldNA, WilletAH, CollierSE, OhiMD, JacksonLP, GouldKLCell reports (2020) 3312: 108526. 710006AT1 ICC; tested species: fission yeast
Analysis of CA Content and CPSF6 Dependence of Early HIV-1 Replication Complexes in SupT1-R5 Cells.ZilaV, MüllerTG, LaketaV, MüllerB, KräusslichHGmBio (2019) 106: . 710006AT1 ICC; tested species: human
Reversible supramolecular assembly of velvet worm adhesive fibers via electrostatic interactions of charged phosphoproteins.BaerA, HänschS, MayerG, HarringtonMJ, SchmidtSBiomacromolecules (2018) : . 710006AT1 ICC
Nanoscale architecture of the Schizosaccharomyces pombe contractile ring.McDonaldNA, LindAL, SmithSE, LiR, GouldKLeLife (2017) 6: . 710006AT1 ICC
An apicosome initiates self-organizing morphogenesis of human pluripotent stem cells.TaniguchiK, ShaoY, TownshendRF, CortezCL, HarrisCE, MeshinchiS, KalantryS, FuJ, O"SheaKS, GumucioDLThe Journal of cell biology (2017) 21612: 3981-3990. 710006AT1 ICC; tested species: human
Mechanoresponsive lipid-protein nanoglobules facilitate reversible fibre formation in velvet worm slime.BaerA, SchmidtS, HaenschS, EderM, MayerG, HarringtonMJNature communications (2017) 81: 974. 710006AT1 ICC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.ReveloNH, RizzoliSOCurrent protocols in neuroscience (2016) 74: 2.25.1-21. 710006AT1 ICC, IHC
SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis.BaranovMV, ReveloNH, DingjanI, MaraspiniR, TerBeestM, HonigmannA, vandenBogaartGCell reports (2016) 176: 1518-1531. 710006AT1 ICC
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.ReveloNH, KaminD, TruckenbrodtS, WongAB, Reuter-JessenK, ReisingerE, MoserT, RizzoliSOThe Journal of cell biology (2014) 2054: 591-606. 710006AT1 ICC, IHC
Ma2/d promotes myonuclear positioning and association with the sarcoplasmic reticulum.ReuvenyA, ShnayderM, LorberD, WangS, VolkTDevelopment (Cambridge, England) (2018) 14517: . 710006AT1 IHC; tested species: drosophila
Whole-head recording of chemosensory activity in the marine annelid Platynereis dumerilii.ChartierTF, DeschampsJ, DürichenW, JékelyG, ArendtDOpen biology (2018) 810: . 710006AT1 IHC
The Membrane Marker mCLING Reveals the Molecular Composition of Trafficking Organelles.ReveloNH, RizzoliSOCurrent protocols in neuroscience (2016) 74: 2.25.1-21. 710006AT1 ICC, IHC
Disruption of adaptor protein 2μ (AP-2μ) in cochlear hair cells impairs vesicle reloading of synaptic release sites and hearing.JungS, MaritzenT, WichmannC, JingZ, NeefA, ReveloNH, Al-MoyedH, MeeseS, WojcikSM, PanouI, BulutH, et al.The EMBO journal (2015) 3421: 2686-702. 710006AT1 IHC; tested species: mouse
A new probe for super-resolution imaging of membranes elucidates trafficking pathways.ReveloNH, KaminD, TruckenbrodtS, WongAB, Reuter-JessenK, ReisingerE, MoserT, RizzoliSOThe Journal of cell biology (2014) 2054: 591-606. 710006AT1 ICC, IHC
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin.GowrisankaranS, HouyS, DelCastilloJGP, SteublerV, GelkerM, KrollJ, PinheiroPS, SchwittersD, HalbsgutN, PechsteinA, vanWeeringJRT, et al.Nature communications (2020) 111: 1266. 710006AT1 UPTAKE; tested species: mouse
