| Cat.No.710-MCK | Content:710-MCKA: unlabeled mCling, 50nmol (lyophilized)710-MCKB: mCLING purification column710-MCKC: Washing buffer, 10ml710-MCKD: Stop solution, 0.5ml710-MCKE: Elution buffer, 1ml710-MCKF: Neutralization buffer, 0.5ml |
| Storage | All components of the kit are stable at 2-8°C.Shelf life before use: 6months. |
| Applications | ICC:yesIHC:yes |
| Documents | Instruction manual |
| Remarks | Due to the positive charge of mCLING, negatively charged coatings of cover-slips should be avoided. We recommend a positively charged coating like poly-L-lysine (PLL). mCLING is a fixable dye but paraformaldehyde alone is not able to fix this molecule sufficiently. Therefore, a mixture of 4%paraformaldehyde (PFA) and 0.2% glutaraldehyde is strongly advised. For detailed protocols see ReveloNH&RizzoliSO,2016. Since the dye selected for coupling can have an impact on the mCling properties, full functionality of the resulting conjugate cannot be guaranteed. |
| Data sheet | 710-mck.pdf |
