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商品描述
Cat. No.: 702-rbCpH
Amount: 50 µg
Price:450.00€
| Cat.No.702-rbCpH | 50 µg affinity purified Fab fragments, lyophilized. Rabbit serum albumin was added for stabilization. For reconstitution add 50µl H2O to get a 1mg/ml solution of antibody in PBS.Either add 1:1 (v/v) glycerol (if this does not interfere with your experiments!), then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt!Avoid bright light to minimize photo bleeching of the fluorescent dye. |
| Applications | ICC:yes (see remarks) gallery |
| Label | CypHer5E |
| Reactivity | Reacts with: rabbit.Other species not tested yet. |
| Remarks | Antigen-purified primary antibodies are recommended. Unpurified rabbit serum or total IgG preparations may contain considerable amounts of unspecific IgGs that will bind plenty of the rbFab-CpH resulting in a lower overall labelling efficiency. The pH sensitive dye only regaines its fluorescence after the acidification of the targeted cellular compartment.ICC:Pre-incubate the primary antibody with a 2.5-fold excess of Fab-CpH conjugate (e.g., 2 µg of primary antibody with 5 µg of Fab fragments) in a total volume of 10 µl for 30 min at RT in the dark.Adjust the antibody to the desired dilution and immediately use it for your internalization assay. |
| Data sheet | 702-rbcph.pdf |
