Cat.No.224211	100 µg purified IgM, lyophilized. Azide was added before lyophilization. For reconstitution add 100µlH2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications	WB:1 : 1000 up to 1 : 2000 (AP staining)galleryIP:not tested yetICC:1 : 500(see remarks) galleryIHC:1 : 500(see remarks) galleryIHC-P/FFPE:not tested yetEM:yes
Clone	53D3
Subtype	IgM (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Epitop	Epitop: AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813)
Reactivity	Reacts with: rat (P62813), mouse (P62812).Other species not tested yet.
Specificity	Specific for GABA-A receptor α1.
matching control protein/peptide	224-2P
Remarks	ICC:This antibody is PFA fixation sensitive, use only mild fixation (2% PFA).Best results are obtained by application of antibodies on living cells. After washingcells with bound antibodies, they can be fixed and visualized with secondaryreagents. IHC:This antibody requires mild fixation. recommended protocol
Datasheet	224_211.pdf

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