Cat.No.274104 | 100 µl antiserum, lyophilized. For reconstitution add 100µlH2O, then aliquot and store at -20°C until use. |
Applications | WB:1 : 1000 (AP staining)(see remarks) galleryIP:yesICC:1 : 500 up to 1 : 1000galleryIHC:1 : 500(see remarks) galleryIHC-P/FFPE:1 : 500gallery |
Immunogen | Synthetic peptide corresponding to AA 585 to 599 from mouse GABA transporter1 (UniProt Id: P31648) |
Reactivity | Reacts with: rat (P23978), mouse (P31648).Other species not tested yet. |
Specificity | Specific for GAT 1. |
matching control protein/peptide | 274-1P |
Remarks | WB:GAT1 aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. IHC:This antibody requires antigen retrieval with pepsin according to:Lorincz A & Nusser Z (2008).recommended protocol |
Datasheet | 274_104.pdf |