MightyCloningReagentSet(BluntEnd)isdesignedtoallowPCRproductstobequicklyclonedintoblunt-endvectorswithasimplemethod.Thebluntingand5"-phosphorylationreactionsaredonesimultaneouslyandinparallel,sobothblunt-and-dAendedproductscanbepreparedinasinglereaction.Thiskiteliminatestheneedforpretreatment(suchasenzymeinactivation,removalofunuseddNTPsandprimers,etc.)ofPCRproducts.