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商品描述
| Cat.No.224211 | 100 µg purified IgM, lyophilized. Azide was added before lyophilization. For reconstitution add 100µlH2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
| Applications | WB:1 : 1000 up to 1 : 2000 (AP staining)galleryIP:not tested yetICC:1 : 500(see remarks) galleryIHC:1 : 500(see remarks) galleryIHC-P/FFPE:not tested yetEM:yes |
| Clone | 53D3 |
| Subtype | IgM (κ light chain) |
| Immunogen | Synthetic peptide corresponding to AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813) |
| Epitop | Epitop: AA 28 to 43 from rat GABA-A receptor α1 (UniProt Id: P62813) |
| Reactivity | Reacts with: rat (P62813), mouse (P62812).Other species not tested yet. |
| Specificity | Specific for GABA-A receptor α1. |
| matching control protein/peptide | 224-2P |
| Remarks | ICC:This antibody is PFA fixation sensitive, use only mild fixation (2% PFA).Best results are obtained by application of antibodies on living cells. After washingcells with bound antibodies, they can be fixed and visualized with secondaryreagents. IHC:This antibody requires mild fixation. recommended protocol |
| Datasheet | 224_211.pdf |
