iMatrix-511 SILK
Laminin 511 is known to bind to Intergin α6β1 of the cell surface.
iMatrix-511 SILK is recombinant human Laminin 511-E8 fragments expressed by transgenic silkworm cocoon.
Product Size : 175ug (175ug x 1 pcs.)
<Application>
iMatrix-511 SILK can be used as cell culture matrix for various cell types including ES/iPS cells.
<Contents>
Liquid Solution (175ug/vial @0.5mg/mL Solvent: PBS(-))
**Specification **
Format: Solution (175ug/vial @0.5mg/mL)
Storage temperature: 2-15°C
<Activity>
The dissociation constant of the binding activity with integrin α6β1 is under 10nM.
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<Procedure>
1. Dilute the solution with sterile PBS (-). Coat dishes with 0.5ug/cm². Add 2mL of diluted iMatrix-511 solution to the well.
* The optimum coating concentration depends on cell lines from 0.1 to 1.5ug/cm². (For example, when you use 6-well plate (9.6 cm²/well), add 9.6ul iMatrix-511 (4.8ug) in 1.99ml of PBS(-).)
2. Incubate for 1 hour at 37°C, 3 hours at room temperature, or over night at 4°C.
3. Remove remaining fluid from the coated surface. No rinse is needed.
4. Immediately plate the cells at desired density.
*** Don't allow the plate to dry.
**** Briefly spin down all liquid in the tube before use.
***** Avoid repeated freeze-thaw cycles.
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****When culturing ES/iPS cell, Feeder Free and Single cell subculture can be done using iMatrix-511.*****
*License to manufacture and sell this product has been obtained from Osaka University & Kyoto University.*
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[Reference Literature]
1. Ido H et al. J. Biol. Chem. 282(15):11144-54, 2007
2. Taniguchi Y et al. J Biol. Chem. 284 (12): 7820-31, 2009
3. Miyazaki T et al. Nat. Commun. 3 1236, 2012
4. Nakagawa M et al. Sci. Rep. 4 3594, 2014
5. Doi D et al. Stem Cell Reports. 2(3):337-50,2014
6. Takashima Y et al. Cell. 158 (6): 1254-69,2014
7. Fukuta M et al. PLoS One. 9(12):e112291,2014
8. Burridge PW et al. Nat. Methods. 11 855-60, 2014
9. Okumura N et al. Invest Ophthalmol Vis Sci. 56(5), 2933-42, 2015
10. Sasaki K et al. Nature 531,367-80, 2016
11. Matsuno Ket al.Differentiation 2016.04.002
12. Nishimura Ket al. Stem Cell Reports 6 (4): 511-524, 2016
13. Takayama K et al. Biochemical and Biophysical Research Communications 474 (1): 91-96, 2016
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