iMatrix-511

Laminin511isknowntobindtoInterginα6β1ofthecellsurface.

iMatrix-511ishighlypurifiedhumanLaminin511-E8fragmentsrecombinantlyexpressedbyCHO-Scell.

ProductSize:175ug (175ugx1pcs.)

<Application>

iMatrix-511canbeusedascellculturematrixforvariouscelltypesincludingES/iPScells.

<Contents>

LiquidSolution(175ug/vial@0.5mg/mLSolvent:PBS(-)) .

**Specification**
Format:Solution(175ug/vial@0.5mg/mL)
Storagetemperature:2-15°C
NaClconcentration:137mM

<Activity>

Thedissociationconstantofthebindingactivitywithintegrinα6β1 isunder10nM.

-------------------------------------------------------------------------------

<Procedure>

1. DilutethesolutionwithPBS(-).Coatdisheswith0.5ug/cm².

*Theoptimumcoatingconcentrationdependsoncelllinesfrom0.1to1.5ug/cm².

**Forexample,whenyouuse6-wellplate(9.6cm²/well),add9.6uliMatrix-511(4.8ug)in1.99mlofPBS(-).Add2mLofdilutediMatrix-511solutiontothewell.

2.Incubatefor1hourat37C,3hoursatRoomtemperature,orovernightat4C.

3.Removeexcessfluidfromthecoatedsurface.Norinseisneeded.

4.Immediatelyplatethecellsatdesireddensity.

***Don"tallowtheplatetodry.

****Brieflyspindownallliquidinthetubebeforeuse.

*****Avoidrepeatedfreeze-thawcycles.

------------------------------------------------------------------------------------------------
****WhenculturingES/iPScell,FeederFreeandSinglecellsubculturecanbedoneusingiMatrix-511.*****

*LicensetomanufactureandsellthisproducthasbeenobtainedfromOsakaUniversity&KyotoUniversity.*

___________________________________________________________________________________________________

[ReferenceLiterature]
1.Miner,JHandYurchenco,PDLamininfunctionsintissuemorphogenesis.Annu.Rev.CellDev.Biol.20,255-284(2004).

2.IdoH,HaradaK,FutakiS,HayashiY,NishiuchiR,NatsukaY,LiS,WadaY,CombsAC,ErvastiJMandSekiguchiK.Moleculardissectionofthealpha-dystroglycan-andintegrin-bindingsiteswithintheglobulardomainofhumanlaminin-10.J.Biol.Chem.279,10946-10954(2004).

3.IdoH,NakamuraA,KobayashiR,ItoS,LiS,FutakiSandSekiguchiK.Therequirementoftheglutamicacidresidueatthethirdpositionfromthecarboxylterminiofthelaminingammachainsinintegrinbindingbylaminins.J.Biol.Chem.282,11144-11154(2007).

4.TaniguchiY,IdoH,SanzenN,HayashiM,Sato-NishiuchiR,FutakiSandSekiguchiK.TheC-terminalregionoflamininbetachainsmodulatestheintegrinbindingaffinitiesoflaminins.J.Biol.Chem.284,7820-7831(2009).

5.MiyazakiT,FutakiS,SuemoriH,TaniguchiY,YamadaM,KawasakiM,HayashiM,KumagaiH,NakatsujiN,SekiguchiKandKawaseE.LamininE8fragmentssupportefficientadhesionandexpansionofdissociatedhumanpluripotentstemcells.Nat.Commun.3,1236(2012).

6.NakagawaM,TaniguchiY,SendaS,TakizawaN,IchisakaT,AsanoK,MorizaneA,DoiD,TakahashiJ,NishizawaM,YoshidaY,ToyodaT,OsafuneK,SekiguchiK,YamanakaS.Anovelefficientfeeder-freeculturesystemforthederivationofhumaninducedpluripotentstemcells.SCIENTIFICREPORTS|4:3594|DOI:10.1038/srep035942014Jan.8

7.YasuhiroTakashima,GeGuo,RemcoLoos,JenniferNichols,GabriellaFicz,FelixKrueger,DavidOxley,FatimaSantos,JamesClarke,WilliamMansfield,WolfReik,PaulBertone,AustinSmith ResettingTranscriptionFactorControlCircuitrytowardGround-StatePluripotencyinHuman. CellVolume158,Issue6,p1254–1269,11September2014DOI:10.1016/j.cell.2014.08.029

8.DoiDetal.IsolationofHumanInducedPluripotentStemCell-DerivedDopaminergicProgenitorsbyCellSortingforSuccessfulTransplantation.
StemCellReports.2(3):337-50,2014

9.TakashimaYetal.ResettingTranscriptionFactorControlCircuitrytowardGround-State
PluripotencyinHuman.Cell.158(6):1254-69,2014

10.FukutaMetal.Derivationofmesenchymalstromalcellsfrompluripotentstemcellsthroughaneuralcrestlineageusingsmallmoleculecompoundswithdefinedmedia.
PLosOne.9(12):e112291,2014

11.Hayashietal.Structure-baseddiscoveryofNANOGvariantwithenhancedpropertiestopromoteself-renewalandreprogrammingofpluripotentstemcells. PNASvol.112(15):4666-4671,April14,2015

FORRESEARCHUSEONLY,NOTFORUSEINDIAGNOSTICPROCEDURES.

Manufacturedby:Nippi,Inc.

Questionsaboutthisproduct?Readytoplaceanorder?Emailusatorders@iwai-chem.comorgiveusacall:(650)486-1541

Iwai-Chem我们的合作伙伴公司Bio Matrix Research，Inc.（BMR）正在申请专利，CELIXSYS™是一种新颖的单克隆抗体筛选方法（免疫沉淀等效系统），可保持抗原构象完整且表位不受空间位阻。  通过克服传统的基于固相的筛选方法（例如抗原包被的ELISA，斑点印迹或蛋白质印迹）的缺点，CELIXSYS™使我们能够获得适用于各种应用的卓越单克隆抗体，例如高灵敏度夹心ELISA，免疫色谱法或抗体阵列系统，以及免疫沉淀和蛋白质印迹。CELIXSYS™使得开发有益的单克隆抗体成为可能，而迄今为止，传统方法尚无法实现。展望未来，这项新技术的应用领域将继续从基础研究和临床诊断试剂扩展到抗体药物的探索。