| ProductName | Cat# | Clone# | Isotype | Applications | Reactivity | Unit/Size |
| AntiMouseG9a/Ehmt2 | PP-A8620A-00 | A8620A | IgG2a | EL,WB,IP,IHC | Hu | 100ug/0.1ml |
*Immunohistochemistry
WTmouse/EScell(Positive)
*Description
HistoneH3Lys9(H3-K9)methylationisacrucialepigeneticmarkfortranscriptionalsilencing.G9aisthemajormammalianH3-K9methyltransferasethattargetseuchromaticregionsandisessentialformurineembryogenesis.
SpecSheetPP-A8620A-00(PDFFile)
| Alias | G9a,Euchromatichistone-lysineN-methyltransferase2,H3-K9-HMTase |
| GeNEBank | AB077209 |
| Nomenclature | |
| Origin | ProducedinBALB/cmouseascitesafterinoculationwithhybridomaofmousemyelomacells(NS-1)andspleencellsderivedfromaBALB/cmouseimmunizedwithBaculovirus-expressedrecombinantmouseG9a(15-114aa). |
| Specificity | ThisantibodyspecificallyrecognizesmouseG9aandcrossreactswithhumanG9a.Notyettestedinotherspecies. |
| Purification | Ammoniumsulfatefractionation |
| Formulation | Physiologicalsalinewith0.1%NaN3asapreservative. |
| Storage | Storeat2C-8Cuptoonemonth.Forlong-termstorage,thesolutionmaybefrozeninworkingaliquots.Repeatedfreezingandthawingisnotrecommended.Storageinafrost-freefreezerisnotrecommended. |
| Reference | TachibanaM.etal.,GenesDev,1;19(7):815-26,2005. |
| Note | Sodiumazidemayreactwithleadandcopperplumbingtoformexplosivemetalazides.Flushwithlargeamountsofwaterduringdisposal. |
Cautionmustbetakentoavoidcontactwithskinoreyes.Insuchacase,rinsethoroughlyatoncewithwater.Donotingest,inhale,orswallow.Seekmedicalattentionimmediately.Wearappropriateprotectiveclothingsuchaslaboratoryoveralls,safetyglassesandgloves.Itisstronglyadvisedthatthisproductshouldbehandledbypeoplewhohavebeenwelltrainedinlaboratorytechniquesandthatitishandledwithcarepursuanttotheprinciplesofgoodlaboratorypractice.Allchemicalsaredeemedpotentiallyharmful.Thevialispronetofallover.Usecaution,especiallywhenthelidisoff.
FORRESEARCHUSEONLY,NOTFORUSEINDIAGNOSTICPROCEDURES.
*Remark-Icon:
EL=ELISA,WB=WesternBlotting,NW=Non-reducingWesternBlotting,S=SuperShiftAssay.IP=Immunoprecipitation,IHC=Immunohistochemistory,CH=ChIPAssay
Hu=Human,Mo=Mouse,Ra=Rat
Manufacturedby:PerseusProteomics,Inc.
Questionsaboutthisproduct?Readytoplaceanorder? |
