| Description | Enzyme Immunoassay for the determination of Bb. |
| Method Type | Competitive-ELISA |
| Sensitivity | 0.19 μg/mL |
| Assay Range | 0.31~20 μg/mL |
| Other Name | |
| Principle | This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Bb. During the reaction, Bb in the sample or standard competes with a fixed amount of Bb on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bb. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated.Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BbT in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Intended Use | This ELISA kit can be applied to the in vitro quantitative determination of Bb concentrations in serum, plasma and other biological fluids. |
| Specificity | This kit recognizes some recombinant and natural Bb. No significant cross-reactivity or interference was observed. |
| Sample Type | Serum, plasma and other biological fluids |
| Kit Components | Micro ELISA Plate, 8 x 12 well strips Reference Standard, 2 vials Concentrated Biotinylated Detection Ab(100x), 120 µL Concentrated HRP Conjugate(100x), 120 µL Reference Standard and Sample Diluent, 20 mL Biotinylated Detection Ab Diluent, 14 mL HRP Conjugate Diluent, 14 mL Concentrated Wash Buffer (25x), 30 mL Substrate Reagent, 10 mL Stop Solution, 10 mL Plate Sealer, 5 pieces Product Manual Certificate of Analysis |
| Other supplies required | Microplate reader with 450nm wavelength filter High-precision transferpettor, EP tubes and disposable pipette tips 37°C Incubator Deionized or distilled water Absorbent paper Loading slot for Wash Buffer |
| Storage | All reagents in this kit should be stored in accordance with the information on product labels. Unused wells should be returned to the foil pouch with the desiccant pack and resealed until air-tight. The Substrate Reagent should not be kept at -20°C. Avoid exposing reagents to strong light during storage or the incubation process. Caps of reagents should be kept tightly sealed to prevent evaporation and microbial contamination. Erroneous results may occur if reagents are not stored as advised above. |
Data Sheet E-EL-0076 (PDF File)
Caution must be taken to avoid contact with skin or eyes. In such a case, rinse thoroughly at once with water. Do not ingest, inhale, or swallow. Seek medical attention immediately. Wear appropriate protective clothing such as laboratory overalls, safety glasses and gloves. It is strongly advised that this product should be handled by people who have been well trained in laboratory techniques and that it is handled with care pursuant to the principles of good laboratory practice. All chemicals are deemed potentially harmful. Vials are prone to fall over. Use caution, especially when the lid is off .
FOR RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Manufactured by : Wuhan Elabscience Biotechnology Co. , Ltd
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