| Description | EnzymeImmunoassayforthedeterminationof3-NT. |
| MethodType | Competitive-ELISA |
| Sensitivity | 0.94ng/mL |
| AssayRange | 1.56~100ng/mL |
| OtherName | 3-Nitro-L-Tyrosine,Nitrotyrosine |
| Principle | ThisELISAkitusesCompetitive-ELISAasthemethod.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwith3-NT.Duringthereaction,3-NTinthesampleorstandardcompeteswithafixedamountof3-NTonthesolidphasesupporterforsitesontheBiotinylatedDetectionAbspecificto3-NT.Excessconjugateandunboundsampleorstandardarewashedfromtheplate,andAvidinconjugatedtoHorserADIshPeroxidase(HRP)areaddedtoeachmicroplatewellandincubated.ThenaTMBsubstratesolutionisaddedtoeachwell.Theenzyme-substratereactionisterminatedbyaddingStopSolutionandthecolorchangeismeasuredspectrophotometricallyatawavelengthof450nm±2nm.Theconcentrationof3-NTTinthesamplesisthendeterminedbycomparingtheODofthesamplestothestandardcurve. |
| IntendedUse | ThisELISAkitcanbeappliedtotheinvitroquantitativedeterminationof3-NTconcentrationsinserum,plasmaandotherBIOLOGicalfluids. |
| Specificity | Thiskitrecognizessomerecombinantandnatural3-NT.Nosignificantcross-reactivityorinterferencewasobserved. |
| SampleType | Serum,plasmaandotherbiologicalfluids |
| KitComponents | MicroELISAPlate,8x12wellstrips ReferenceStandard,2vials ConcentratedBiotinylatedDetectionAb(100x),120µL ConcentratedHRPConjugate(100x),120µL ReferenceStandardandSampleDiluent,20mL BiotinylatedDetectionAbDiluent,14mL HRPConjugateDiluent,14mL ConcentratedWashBuffer(25x),30mL SubstrateReagent,10mL StopSolution,10mL PlateSealer,5pieces ProductManual CertificateofAnalysis |
| Othersuppliesrequired | Microplatereaderwith450nmwavelengthfilter High-precisiontransferpettor,EPtubesanddisposablePipettetips 37°CIncubator Deionizedordistilledwater Absorbentpaper LoadingslotforWashBuffer |
| Storage | Allreagentsinthiskitshouldbestoredinaccordancewiththeinformationonproductlabels.Unusedwellsshouldbereturnedtothefoilpouchwiththedesiccantpackandresealeduntilair-tight.TheSubstrateReagentshouldnotbekeptat-20°C.Avoidexposingreagentstostronglightduringstorageortheincubationprocess.Capsofreagentsshouldbekepttightlysealedtopreventevaporationandmicrobialcontamination.Erroneousresultsmayoccurifreagentsarenotstoredasadvisedabove. |
DataSheetE-EL-0040(PDFFile)
Cautionmustbetakentoavoidcontactwithskinoreyes.Insuchacase,rinsethoroughlyatoncewithwater.Donotingest,inhale,orswallow.Seekmedicalattentionimmediately.Wearappropriateprotectiveclothingsuchaslaboratoryoveralls,safetyglassesandgloves.Itisstronglyadvisedthatthisproductshouldbehandledbypeoplewhohavebeenwelltrainedinlaboratorytechniquesandthatitishandledwithcarepursuanttotheprinciplesofgoodlaboratorypractice.Allchemicalsaredeemedpotentiallyharmful.Vialsarepronetofallover.Usecaution,especiallywhenthelidisoff.
FORRESEARCHUSEONLY,NOTFORUSEINDIAGNOSTICPROCEDURES.
Manufacturedby: WuhanElabscience BiotechnologyCo. ,Ltd
Questionsaboutthisproduct? Readytoplaceanorder? |
