NucleoMag VET enables manual or automated purification of viral RNA and DNA and bacterial DNA using proven NucleoMag magnetic bead technology. The kit is designed for common veterinary sample material, such as animal whole blood, serum/plasma, feces, ear notches, tissue, or swabs. After a sample-specific pre-lysis procedure, microbial nucleic acids are purified from 200µl of liquid or homogenized sample. The entire NucleoMag VET protocol can be automated on common liquid handling platforms.
NucleoMag VET enables manual or automated purification of viral RNA and DNA and bacterial DNA using proven NucleoMag magnetic bead technology. The kit is designed for common veterinary sample material, such as animal whole blood, serum/plasma, feces, ear notches, tissue, or swabs. After a sample-specific pre-lysis procedure, microbial nucleic acids are purified from 200µl of liquid or homogenized sample. The entire NucleoMag VET protocol can be automated on common liquid handling platforms.
The NucleoMag principle is similar to silica membrane technology: nucleic acids are bound to magnetic beads under chaotropic salt conditions, contaminants are washed away with ethanolic wash buffers, and pure RNA/DNA are eluted from the beads using elution buffer. NucleoMag technology can be used either with static-pin separators (e.g., NucleoMag SEP) or with magnetic separators integrated into robotic workstations.
Figure 1. Sensitive detection of viral DNA from animal tissue samples. Viral DNA was purified from PCV2-positive animal tissue samples (n = 22, 20 mg each) using NucleoMag VET and a kit from Competitor Q, respectively, and analyzed by qPCR. Of the 22 samples tested, 20 were found to be positive for PCV2 using NucleoMag VET, whereas only 14 were found to be positive using the kit from Competitor Q. The graph depicts mean CT values for amplification of the PCV2 sequence from viral DNA purified with either kit.
Figure 2. Sensitive detection of viral RNA and DNA from animal blood samples. Purified Viral RNA and DNA obtained from 200 µl blood serum samples of PRRSV- and PCV2-positive animals using NucleoMag VET was diluted 10-fold and analyzed by qPCR. For both RNA and DNA (blue and gray bars, respectively), CT values correlate with dilution factor. Sequences corresponding to PRRSV and PCV2 were detectable across the range of dilutions tested.
