The NucleoSpin RNA Clean-up kits provide a simple, fast, and convenient way to clean up your RNA from prepurified RNA (phenol/chloroform), enzymatic reactions (e.g, amplification or labeling reactions), DNase digestions, and more. The kits enable complete removal of RT-PCR inhibitors, leading to superior RT-PCR results. The time-saving procedure is based on the protocol employed by the NucleoSpin RNA purification kits, excluding the DNase digestion and homogenization steps.
The NucleoSpin RNA Clean-up kits provide a simple, fast, and convenient way to clean up your RNA from prepurified RNA (phenol/chloroform), enzymatic reactions (e.g, amplification or labeling reactions), DNase digestions, and more. The kits enable complete removal of RT-PCR inhibitors, leading to superior RT-PCR results. The time-saving procedure is based on the protocol employed by the NucleoSpin RNA purification kits, excluding the DNase digestion and homogenization steps.
NucleoSpin RNA Clean-up is also recommended for RNA isolation from cultured cells whenever copurification of some genomic DNA is acceptable. For isolating RNA from cells and tissue with the least DNA contamination, we recommend the rDNase-containing NucleoSpin RNA purification kits. These kits enable preparation of pure RNA with an A260/280 ratio generally exceeding 1.9 (measured in TE buffer, pH 7.5).
RNA purified with the NucleoSpin RNA Clean-up kits is ready to use in applications such as enzymatic labeling reactions (including dye incorporation), reverse transcriptase-PCR (RT-PCR), and DNA/RNA-based chip hybridizations. The NucleoSpin RNA Clean-up kits can also be used to clean up in vitro-transcribed RNA and aminoallyl mRNA, as well as biotinylated and fluorescently labeled RNA.
