NucleoBond PC EF Kits use anion exchange and gravity flow for purification of endotoxin-free plasmid DNA. Endotoxin removal is achieved in a unique washing step—no extra incubations are necessary.Lysates are cleared by simple filtration using the included NucleoBond Folded Filters—no centrifugation is required. NucleoBond PC 2000 EF is used to purify 500–2000 µg of endotoxin-free plasmid DNA.