Direct One-Step RT-qPCR Mix for SARS-CoV-2 enables accurate one-step RT-qPCR on unpurified saliva samples to detect the presence and amount of virus. No RNA extraction step is needed; just mix the sample and pretreatment reagent, heat treat, and then combine with the RT-qPCR master mix. The whole protocol, from pretreatment through detection, takes less than one hour, enabling fast time to results. This protocol provides guidelines for multiplexed SARS-CoV-2 detection in saliva samples, but no primers are included (see Overview section below for more information).