Antibiotics for the selection and maintenance of eukaryotic cells that are stably transfected with vectors expressing neomycin, puromycin, and hygromycin resistance cassettes.

G418, Puromycin, & Hygromycin B dose concentrations for selection & maintenance

For selecting colonies, the appropriate dose must be determined empirically for each specific cell line. We recommend testing a dosage range using dishes of untransfected cells and choosing the dose that kills all of the cells in 3–5 days. If all of the cells die in less than 24 hours, you should use a lower dose.