Takara Bio offers both monoclonal and polyclonal antibodies raised against recombinant Cas9 protein from Streptococcus pyogenes.The Guide-it Cas9 Monoclonal Antibody (Clone TG8C1) is a mouse antibody produced by hybridoma cells against full-length recombinant Cas9 protein from Streptococcus pyogenes. This antibody can recognize less than 1 ng of wild-type Cas9 in bacterial and mammalian cell lysates by Western blot.

The Guide-it Cas9 Polyclonal Antibody is a rabbit antibody raised against recombinant Cas9 protein from Streptococcus pyogenes. This antibody recognizes three variants of the Cas9 nuclease: wild-type Cas9, the Cas9 nickase, and Cas9 nuclease-deficient mutants. The antibody is specific and sensitive, no background expression is detected in most mammalian cell lines, and the antibody can recognize as little as 0.5 ng of recombinant Cas9 by Western blot.