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Cat.No.152503 | 50µgspecificantibody,lyophilized.Affinitypurifiedwiththeimmunogen.AlbuminwasaddedforstABIlization.Forreconstitutionadd50µlH2Otogeta1mg/mlsolutioninPBS.Thenaliquotandstoreat-20°Cuntiluse. |
Applications | WB:1:1000(APstaining)(seeremarks)galleryIP:nottestedyetICC:nottestedyetIHC:1:200upto1:500galleryIHC-P/FFPE:nottestedyet |
Immunogen | RecombinantproteincorrespondingtoAA1921to2254fromratCa2+channelT-typeα-1G(UniProtId:O54898) |
Reactivity | Reactswith:rat(O54898),mouse(Q9WUT2).Otherspeciesnottestedyet. |
Specificity | SpecificforCa2+channelα-1G. |
Remarks | WB:Duetoitslargesize,thisantibodyrequiresspecialgel-electrophoresisandWesternblotprotocolsforvisualizationbyimmunoblotting.Excellentresultscanbeobtainedwiththe4-12%TRIS-glycinegrADIentgelsofanamedorNuPageTRIS-acetategelsfromInvitrogen.Thisproteintendstoaggregateafterboiling,makingitnecessarytorunSDS-PAGEwithnon-boiledsamples. |
Datasheet | 152_503.pdf |