GAL4 AD and GAL4 DNA-BD Monoclonal Antibodies bind specifically to the major activation domain (AD) of GAL4 yeast two-hybrid prey proteins, and the DNA-binding domain (DNA-BD) of the bait protein, respectively.

If your bait vector is pGBKT7, your fusion protein can be detected with the GAL4 DNA-BD Monoclonal Antibody (our monoclonal c-Myc Antibody may also be used).

If your prey vector is pGADT7, pGADT7-Rec, or pACT2, the prey fusion protein can be detected with the GAL4 AD Monoclonal Antibody (our polyclonal HA-Tag Antibody may also be used).

These antibodies are ideal for:

• Confirming that a GAL4 fusion protein is being expressed and has the expected molecular weight
• Characterizing putative interactors with your protein of interest
• Co-immunoprecipitation studies