Our yeast two-hybrid media sets contain all of the yeast media pouches you will need for the Matchmaker Gold Yeast Two-Hybrid System protocols. The media is sold aliquoted in highly convenient pouches. Simply empty the contents of each pouch into your media flask/bottle, add 0.5 L of dH₂0, and sterilize. No weighing, combining, or pH adjustment is required. The Yeast Media Set 2 Plus also contains Aureobasidin A and our largest aliquot of X-alpha-Gal, two reagents that are integral to the Matchmaker Gold Protocol.