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Clontech/Yeast one-hybrid system—Matchmaker Gold/5 Rxns/630491

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货号:630491
浏览量:127
品牌:Clontech
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商品描述

Our Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbAr) as a stringent, highly selective, and easy-to-use reporter. This novel reporter produces very low screening backgrounds, since the Aureobasidin A antibiotic (AbA) efficiently kills yeast lacking AbAr expression.

Our Matchmaker Gold Yeast One-Hybrid Library Screening System provides a simple and efficient method for identifying and characterizing novel protein-DNA interactions. All Matchmaker Gold Systems use Aureobasidin A resistance (AbAr) as a stringent, highly selective, and easy-to-use reporter. This novel reporter produces very low screening backgrounds, since the Aureobasidin A antibiotic (AbA) efficiently kills yeast lacking AbAr expression.

The Matchmaker Gold Yeast One-Hybrid Library Screening System

In the Matchmaker Gold Yeast One-Hybrid Library Screening System, tandem repeats of your DNA target/bait sequence are cloned into the pAbAi reporter vector. To generate your bait-specific reporter strain, the pAbAi vector is then integrated into the genome of Y1HGold yeast using homologous recombination. This strain provides a host for library screening.

One-Step library construction and screening

A cDNA library of potential DNA-binding proteins, which are ultimately expressed as fusions to the yeast GAL4 transcription activation domain (GAL4 AD prey proteins), is constructed directly in the Y1HGold[Bait-AbAi] reporter strain using SMART technology and homologous recombination. When a prey protein binds to the bait sequence, its associated GAL4 AD activates AbAr expression, allowing the cell to grow on medium containing AbA. In library screens, the plasmids encoding the library-derived prey proteins can be easily rescued from the surviving yeast clones and subjected to further analysis.

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