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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
D-Glucose | 3151 mg/L |
---|---|
L-Glutamine | 365 mg/L |
HEPES | No HEPES |
Sodium Pyruvate | 55 mg/L |
Phenol Red | 8.1 mg/L |
Since Harry Eagle"s first reports, studies have been underway to determine the media requirements for individual cell types. The medium found to be most satisfactory for these studies as a 1:1 mixture of Dulbecco"s Modified Eagle"s Medium (DMEM) and Ham"s F-12 Nutrient Mixture. To help compensate for the buffering capacity lost with the removal of serum, HEPES has been added in some modifications at a final concentration of 15 mM.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。