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品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
| Components | - PURY Cell & Tissue Lysis(PCT) Buffer- PURY Wash Buffer 1- PURY Wash Buffer 3- RNase Free Water- PURY RNA Mini Column(blue ring) in Collection Tube- gDNA Removal Column(yellow ring) in Collection Tube- Collection tubes |
|---|---|
| Quality Control | In accordance with GenDEPOT’s Quality Assurance System, each lot of PURY RNA Plus is tested against predetermined specifications to ensure consistent product quality. |
| Downstream Applications | The purified RNA is ready to use and is ideally suited for downstream applications that are sensitive to low amounts of DNA contamination, such as quantitative,real-time RT-PCR. The purified RNA can also be used in other applications, including: RT-PCR, Differential display, cDNA synthesis, Northern, dot blot, slot blot analyses, Primer extension, Poly A+ RNA selection, RNase/S1 nuclease protection, Microarrays. |
The PURY RNA Plus is designed to purify RNA from small amounts (up to 1X107 cells) of a variety of cells and easy-to-lyse tissue types. This kit suppliesthe gDNA Removal Columns, a spin column that quickly and effectively removesgenomic DNA without the need of DNase digestion. One of the most importantaspects during the isolation of RNA is to prevent degradation of the RNA. Cellsand tissues are first lysed by incubation in a chaotropic ion PURY Cell & Tissue LysisBuffer, which immediately inactivates RNases. The lysate is added to the gDNARemoval Column (yellow rings) to clarify the lysate and to remove contaminatinggDNA. After the addition of ethanol with the flow-through, the RNA is bound tothe Pury RNA Mini Column (blue rings). Subsequent wash steps removes salts,metabolites, and macromolecular cellular components. High quality RNA is elutedwith RNase-free H2O. The RNA preparation using PURY RNA Mini Plus can be
performed at room temperature.
