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Gendepot/MTT Assay Reagent, Ready-to-Use, M0200/10x1 ml/M0200-010

价格
¥1900.00
货号:M0200-010
浏览量:127
品牌:Gendepot
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商品描述
The MTT Assay Reagent provides a simple method for determining live cellnumbers using a standard colorimetric plate readers. Determination of live cellnumbers is often used to assess the rate of cell proliferation and cytotoxicitycaused by drugs and cytotoxic agents. Among all non-radioactive viability assays,MTT assay developed by Mossman is one of the most versatile and popular assays.
MTT is a tetrazolium salt that is turned into a purple formazan product afterreduction by mitochondrial enzymes that are only present in metabolicallyactive live cells, not in dead cells. The amount of formazan product generatedis proportional to the number of living cells in the sample. At the end of the assay,the cells containing the formazan product are solubilized and then photometricallyquantified at 570 nm.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。