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Gendepot/Oligo(dT)12-18 ,O1025/50ug/O1025-050

价格
¥2900.00
货号:O1025-050
浏览量:127
品牌:Gendepot
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商品描述
ApplicationcDNA Synthesis
RT-qPCR
RT-PCR
Oligo d(T)12-18 is the classic primer mix used to prime synthesis of the first strand cDNA by reverse transcriptase using poly A+ mRNA as a template. Oligo dT of various sizes are synthesized individually and gel purified. Oligo d(T)12-18 is a mixture of individually synthesized and purified primers of varying sizes. These are mixed in equimolar ratio. Oligo purity is greater than 98% as determined by denaturing polyacrylamide gel electrophoresis.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。