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商品描述
| Components | 55ml Substrate (5 x 11 vials)3ml AEC Chromogen |
|---|
3-Amino-9-Ethylcarbazole (AEC) is a widely used chromogen for immunohistochemical staining and immunoblotting. When in the presence of peroxidase enzyme , AEC produces a vivid red end product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media. This product is available in a two component form consisting of a liquid, refrigerator stable AEC Chromogen and AEC Substrate. Each component is individually stable for 18 months from the date of manufacture. This kit is formatted using convenient dropper-top vials.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
