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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/Human Transforming Growth Factor-Beta1(TGF-b1), CG116/1 ug/CG116-001

Gendepot/Human Transforming Growth Factor-Beta1(TGF-b1), CG116/1 ug/CG116-001

价格
￥3600.00
货号：CG116-001
浏览量：100
品牌：Gendepot
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商品描述

Transforming growth factor betas (TGFBetas) mediate

many cell-cell interactions that occur during embryonic

development.Three TGFBetas have been identified in

mammals. TGFBeta1,TGFBeta2 and TGFBeta3 are

eachsynthesized as precursor proteinsthat are very

similar inthat each is cleaved to yield a 112 amino

acidpolypeptidethat remains associated with the

latentportion of themolecule.TGFB1 Human

Recombinantproduced inHuman 293 cells is a

homodimeric pol-peptide chaincontaining 2 x 112

amino acids andhaving a totalmolecular mass of

25kDa. The TGFB1is purified byproprietary

chromatographic techniques.

SourceHuman 293 cells

Physical DescriptionSterile Filtered White lyophilized (freeze-dried) powder

FormulationLyophilized from a concentrated (1mg/ml) solution

containing 10mM HCl & 50ug BSA

SolubilityIt is recommended to reconstitute the lyophilized

TGFB1in sterile 5mM HCl & 50ug/ml BSA at a

concentration of0.1mg/ml, which can then be further

diluted to otheraqueous solutions

Store at -20oCLyophilized form stable at room temperature

for 3 weeks,should be stored desiccated below

-20oC .Upon reconstitution, should be stored at 4oC

between 2-7 daysand for future use below -20oC

Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。