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Gendepot/Epidermal Growth Factor,Human Recombinant, Pichia , CG016/100 ug/CG016-100

价格
¥3600.00
货号:CG016-100
浏览量:127
品牌:Gendepot
服务
全国联保
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商品描述
SourcePichia Pastoris.
FormulationEach lyophilized mg contains 0.15M NaCl, 0.025M sodium bicarbonate, pH-7.5.
PurityGreater than 95% as obsereved by SDS-PAGE.
Biological ActivityThe ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1="" ng/ml="" corresponding="" to="" a="" specific="" activity="" of="">
StabilityLyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mirogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton. The EGF is purified by proprietary chromatographic techniques.

Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。