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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
| Application | Digestion of DNA in the recovery of intact RNA |
|---|---|
| Digestion of DNA in the recovery of intact Protein | |
| DNA Mapping | |
| Digest DNA from damaged cells | |
| Removing membrane bound DNA fragments |
DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3”-OH
oligonucleotides. Mg²⁺ in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence of Mn²⁺, DNase I cleaves both strands of the DNA.
DNase I is useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. DNase I is a chromatographically pure preparation. It is offered as lyophilized powder. For greatest stability, it is important that DNAse be dissolved at a concentration of at least 1mg/ml in 50% Glycerol with 20mM Tris-HCl, pH 7.5 and 1mM MgCl2. This solution can be stored at -20oC for at least a year.
