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蚂蚁淘在线 / 品牌中心 / Gendepot / Gendepot/IPTG, Isopropyl-beta-D-Thiogalactopyranoside, I0355/2 g/I0355-002
商品描述
| Solubility | Soluble in water and methanol |
|---|---|
| pH 5% in Water | 5.0-7.0 |
| Assay by HPLC | 98-101% w/w (on anhydrous basis) |
IPTG, Dioxane-Free (isopropyl-β-d-thiogalactopyranoside), is an inducer of β-galactosidase activity in many bacteria. Functioning as a lac analog, IPTG induces β-galactosidase activity by binding to and inhibiting the lac repressor. This product is used to differentiate recombinants from nonrecombinants in cloning strategies using vectors containing the lacZ or lacZ α-peptide gene.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接，这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后，可通过在65℃温育10分钟使T4 DNA连接酶失活。 
