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Gendepot/Xpert Plant Protein Extraction Kit , P3113/P3113-050-50 Prep

价格
¥7400.00
货号:P3113-050-50Prep
浏览量:54
品牌:Gendepot
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全国联保
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商品描述
Downstream Application1-D and 2-D gel electrophoresis, Western blotting, activity assays, and protein affinity purifications,LC/MS, IP, ELISA, 2D-gel analysis, isoelectric focusing and other application
Kit ComponentsXpert Plant Lysis Buffer , P3113L , 25 mlXpert Filter Column , P3113C , 50 columnsXpert Collection Tubes with cap, P3113CT , 50 tubesPlastic Rods , 4 rods
Xpert Plant Protein Extraction kit is composed of optimized Plant Lysis Buffer andXpert Filter Column with 2.0 ml collection tubes. The kit is designed to rapidlyextract water soluble proteins from plant tissues. The Xpert Plant Lysis Bufferdoes not contain any detergent and organic solvent. Due to the use of the proteinextraction column, the extraction volume can be as small as 50 μl and as largeas 500 μl.Detergent-free total proteins can be extracted from plant tissues(leaves, seeds, soft stems etc.) in less than 8 min with high yield (1-5 mg/ml).
Extracted detergent free water soluble proteins from plant tissues for applicationssuch as proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing,SDS-PAGE, immunoblottings, and other applications.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。