The Xpert Phosphatase Inhibitor Cocktail Solution(100X)

is a mixture of several inhibitors to prevent valuable

proteinsfrom dephospholylation. Phosphorylation and

dephosphorylation is a molecular on/off switch that regulates

a number of key biological pathways within the cell,

including signal transduction, cell division, and apoptosis.

The Xpert Phosphatase Inhibitor Cocktail preserves

phosphorylation of proteins in cell lysates and tissue

extracts.The cocktail contains a mixture of four

phosphatase inhibitors of broad specificity, including

sodium fluoride, sodium orthovanadate, sodium

pyrophosphateand sodium glycerophosphate.

Unlike other commerciallyavailable phosphatase

inhibitor cocktails which protectagainst either

Ser/Thr phosphatases or protein tyrosine

phosphatases.

The Xpert Phosphatase Inhibitor Cocktail protects

phosphoproteins from both families of phosphatases.

Conveniently packaged in a 100X format, the cocktail

is stable at 4°C and is compatible with common protein

assays, including the BCA Protein Assay and Bradford-

basedassays such as Coomassie.