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Gendepot/Protease Inhibitor Cocktail II, EDTA Free (10X), Tablets, P5101/-

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品牌:Gendepot
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商品描述
Protease Inhibitor Cocktail II, EDTA Free tablets inhibit a broad spectrum of serine and cysteine protease. This inhibitor cocktail do not contain EDTA leaving the stability and the function of metal dependant proteins uneffected. The affinity purification of Poly-His tagged fusionproteins via IMAC (immobilized metailche
-late affinity chromatography) is also facilitated (no dialysis necessary). Due to the optimized composition of the tablets they show excellent inhibition effects and are therefore very well suited for the protection of proteins isolated from animal tissues, plants, yeast and bacteria. Protease Inhibitor Cocktail II, EDTA Free
contains both irreversible and reversible protease inhibitors. Metallo and aspartic proteases are not inhibited. The protease inhibitors of Protease Inhibitor Cocktail II, EDTA Free do not form irreversible complexes with thiol (SH) groups of proteins.
Gendepot的T4 DNA连接酶是最通用的DNA克隆连接酶。这种ATP依赖性酶将5'-磷酸与3'-羟基化的末端共价连接,这些末端是通过限制性酶消化或其他酶促过程产生的双链DNA片段的平末端或相容的内聚末端。T4 DNA连接酶对单链核酸无活性。连接反应后,可通过在65℃温育10分钟使T4 DNA连接酶失活。