Application:FCAdditional Information:Description: Cell Viability Dye Red 710 is an amine reactive viability dye that can be used to discriminate viable from non-viable mammalian cells in flow cytometry applications. This dye irreversibly binds free amines available on the cell surface as well as intracellular free amines exposed in cells with compromised cell membranes. Necrotic cells with compromised membranes will react with significantly more Cell Viability Dye Red 710 dye than viable cells in the same sample and therefore will exhibit much greater fluorescence intensity allowing exclusion of these cells from analysis.:::Excitation Laser: Red (633-640 nm):::Emission: 710 nmReconstitution and Storage:-20C protected from light and moistureApplication Info:Cell Viability Dye Red 710 has been quality-tested for flow cytometry using mouse thymocytes and can be used at 1 uL/mL of cell suspension. This is a very bright dye. To reduce excess spillover the concentration required for optimal performance should be determined empirically by the investigator. Cell Viability Dye Red 710 is excited by the red (633-647 nm) laser line and has a peak emission of 710 nm that can be detected using the recommended 710/50 band pass filter commonly used for detection of Alexa Fluor 700.Formulation:1 uL/test in DMSODatasheets/Manuals:Printable datasheet for OOTA00663 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物,这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌,哺乳动物细胞和昆虫细胞中表达,并作为整体裂解物提供。