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Cell Stimulation Cocktail (500X) is a ready to use cocktail that can be used for polyclonal cell activation. It is composed of the phorbol ester, PMA (Phorbol 12-Myristate 13-Acetate), a calcium ionophore (Ionomycin), and the protein transport inhibitors Brefeldin A and Monensin. This Cell Stimulation Cocktail (500X) can be used to elicit cytokine production and retention in a variety of cell types allowing for detection of intracellular protein by flow cytometry.

Reconstitution and Storage:

-20C, protected from light and tightly sealed to prevent evaporation

Application Info:

Cell Stimulation Cocktail (500X) has been quality-tested by treatment of mouse splenocytes for 6 to 18 hours followed by intracellular staining and flow cytometric analysis of IL-2 or IFN-gamma. Cell Stimulation Cocktail is supplied as a 500X solution and should be used at 1X final concentration for cell stimulation. It is recommended to add the cocktail directly to the cell suspension at 2 uL/mL to obtain a 1X final concentration.

Datasheets/Manuals:

Printable datasheet for OOTA00786

我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。

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