Tested Species Reactivity:HumanPredicted Species Reactivity:Chicken, Mouse, RatProduct Format:LyophilizedApplication:CAAdditional Information:Description: Transforming Growth Factor alpha (TGF-alpha) is a member of the EGF subfamily, shares over 40% identity with EGF, and binds to the EGF receptor (EGFR) to stimulate the proliferation of a wide range of epidermal and epithelial cells. TGF-alpha also has angiogenic properties. Binding to the EGFR activates the PI3K and Erk1/2 pathways. It is expressed by keratinocytes, epithelial cells, macrophages and various tumors and tumor cell lines. Overexpression of TGF-alpha has been linked to a variety of cancers including breast, colon, and stomach cancers.:::Bioactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells is <= 0.2="" ng/ml,="" corresponding="" to="" a="" specific="" activity="" of="">= 5 x 106 units/mg.:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein=""><1>Reconstitution and Storage:-20CPurity:98%Protein Sequence:VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLASource:E. coliTarget Reference:Massagué J. 1990. J Biol Chem. 265(35):21393-21396.
Derynck R, Roberts AB, Winkler ME, Chen EY and Goeddel DV. 1984. 38(1): 287-297.
Groenen LC, Nice EC and Burgess AW. 1994. Growth Factors. 11(4): 235-257.
Lui S, Wierod L, Skarpen E, Grosvik H, Duan and Huitfeldt HS. 2013. Cell Physiol Biochem. 32(3): 511-522.
Schreiber AB, Winkler ME and Derynck R. 1986. Science. 232(4755): 1250-1253.
Kumar V, Bustin SA and McKay IA. Cell Biol Int. 1995. 19(5): 373-388.
Datasheets/Manuals:Printable datasheet for OPTA01065Alias Symbols:Transforming Growth Factor-alpha, Sarcoma growth factor, TGF-type I, ETGFMolecular Weight:5.5 kDa 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
