Tested Species Reactivity:MousePredicted Species Reactivity:Human, Monkey, Rat, CowProduct Format:LyophilizedApplication:CAAdditional Information:Description: TNF-alpha is a clinically important cytokine with strong pro-inflammatory activities. TNF-alpha is secreted by a variety of immune and non-immune cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. TNF-alpha signals through two receptors, TNFR1 and TNFR2. TNF-alpha has strong cytolytic activities against a wide variety of tumor cells and is critically important in combat against bacterial infections. The inflammatory effects of TNF-alpha are also deleterious in causation of septic shock and autoimmune diseases including rheumatoid arthritis, inflammation, and diabetes. :::Bioactivity: ED50 is <= 0.1="" ng/ml,="" corresponding="" to="" a="" specific="" activity="" of="">= 1 x 107 units/mg calculated by the cytolysis of Mouse L929 cells in the presence of actinomycin D.:::Endotoxin Level: Endotoxin level is <0.1 ng/ug="" of="" protein=""><1>Reconstitution and Storage:-20CPurity:98%Protein Sequence:MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIALSource:E. coliTarget Reference:Feldmann M and RN Maini 2010 J Immunol 185: 791-794.
Palladino MA, FR Bahjat, EA Theodorakis and LL Moldawer 2003 Nat Rev Drug Discov 2: 736-46.
Swardfager W, K Lanctôt, L Rothenburg, A Wong, J Cappell and N Herrmann 2010 Biol Psychiatry 68: 930-941.
Locksley RM, N Killeen and MJ Lenardo 2001 Cell 104: 487-501.
Kolb WP and GA Granger Proc Natl Acad Sci USA 61(4): 1250-1255.
Ruddle NH and BH Waksman 1968 J Exp Med 128(6): 1267-1279.
Carswell EA, LJ Old, RL Kassel, S Green, N Fiore and B Williamson 1975 Proc Natl Acad Sci USA 72: 3666-3670.
Datasheets/Manuals:Printable datasheet for OPTA01021Alias Symbols:Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, CytotoxinMolecular Weight:17.3 kDa 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
