Predicted Species Reactivity:HumanApplication:ELISAKit Detection Method:Colorimetric, OD450 nmKit Principle:Aviva Systems Biology KIR2DL3 ELISA Kit (Human) (OKEH07603) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KIR2DL3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KIR2DL3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KIR2DL3 captured in well.Kit Range:0.78 - 50ng/mLKit Recovery:Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 111% (n = 20)Kit Reproducibility:Mean Intra-assay CV%: <=4.6% (n="">Mean Inter-assay CV%: <=9.9% (n="">Kit Component:| Component | Amount |
|---|
| KIR2DL3 Microplate | 96 Wells (12 x 8 Well strips) |
| KIR2DL3 Lyophilized Standard | 2 |
| 100X Biotinylated KIR2DL3 Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Conjugate | 1 x 120 uL |
| Sample Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 25X Wash Buffer | 1 x 30 mL |
| TMB Substrate | 1 x 10 mL |
| Stop Solution | 1 x 10 mL |
Additional Information:Function: Receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). Inhibits the activity of NK cells thus preventing cell lysis.:::Subcellular Location: Cell membrane Single-pass type I membrane protein:::Unigene: Hs.654608SMR: P43628String: 9606.ENSP00000342215:::KEGG: hsa:3804MIM: 604938Pfam: PF00047:::KEGG: hsa:3804MIM: 604938Pfam: PF00047Reconstitution and Storage:Store as indicated in product manual.Sensitivity:0.38ng/mLDatasheets/Manuals:Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.Specificity:Natural and recombinant Human Killer cell immunoglobulin-like receptor 2DL3Assay Info:Assay Methodology: Quantitative Sandwich ELISAGene Symbol:KIR2DL3Official Gene Full Name:killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 3Alias Symbols:p58, NKAT, GL183, NKAT2, CD158b, KIR2DL, NKAT2A, NKAT2B, CD158B2, KIR-K7b, KIR-K7c, KIR2DS5, KIRCL23, KIR-023GBNCBI Gene Id:3804Protein Name:killer cell immunoglobulin-like receptor 2DL3Description of Target:Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response.Swissprot Id:P43628Protein Accession #:NP_056952.2Nucleotide Accession #:NM_015868.2Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express KIR2DL3. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express KIR2DL3. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
