Application:FC,IFAdditionalInformation:TechnicalSummaryAbs/EmMaxima:642/662nmExtinctioncoefficient:105,000Molecularweight:~832Excitationlaserline:633nm,635nmor640nmDirectreplacementfor:AlexaFluor®647,ATTO™647N,Cy™5,DyLight®649:::Advantages:-HasthebestphotostABIlityamongdyeswithCy®5-likespectra-Yieldshighlyfluorescentproteinconjugates-Verywater-solubleandpH-insensitive :::| Component | 50-100ugkit |
| Dyevial | (1)vialComponentA |
| Mix-n-Stainreaction | (1)vialof30uLbuffer,10XPart#99951 |
| Mix-n-Stainantibody | (1)vialof300uLstoragebufferPart#99952 |
| Ultrafiltrationvial(MWCO=10K) | 1eachPart#99956 |
:::Pleasevisitwww.avivasySBIo.comtoviewourfullselectionofproductsfeaturingbrightandphotostableCF™dyes,includingsecondaryantibodies,streptavidin,anti-biotin,andanti-tagantibodies.Avivaalsooffersavarietyofapoptosisandcellviabilityassaysforflowcytometryanalysis,includingmitochondrialmembranepotentialdyes,fluorescentAnnexinVconjugates,andNucView™488Caspase-3Substrateforlivecells.CFdye,Mix-n-Stain,andmodifiedMix-n-StainlabelingtechnologiesarecoveredbypendingUSandinternationalpatents.TWEENisaregisteredtrademarkofUniqemaAmericasLLC.Avivaproductsarehigh-qualityreagentsandmaterialsintendedforresearchpurposesonly.Someproductsarepotentiallyhazardouschemicals-pleasereadtheMaterialSafetyDataSheetforadditionalinformationregardinghandlingpotentiallyhazardouschemicals.SeveralofAvivaproductsandproductapplicationsarecoveredbyU.S.andinternationalpatentsandpendingpatents.OurproductsarenotavailableforresaleorothercommercialuseswithoutaspecificagreementfromAvivaSystemsBIOLOGyCorporation.Wewelcomeinquiriesaboutlicensingtheuseofourdyes,trademarksortechnologies.Pleasesubmitinquiriesbye-mailtoinfo@avivasysbio.com ReconstitutionandStorage:-20CStability:Stableforatleast3monthsfromdateofreceiptwhenstoredasrecommended.Datasheets/Manuals:Printabledatasheet/referencemanualforMix-n-StainCF640RAntibodyLabelingKit,1x(50-100ug)labeling(OKBE00034)OKBE00034DescriptionofTarget:Mix-n-Stain™antibodylabelingkitscontaineverythingyouneedtorapidlylabelanantibodywithAviva"snext-generationCF™dyesorbiotin.Thelabelingprocedurecomprisessimplemixingofyourantibodywiththereactionbufferandoptimallyformulateddyeprovided,followedbyabriefincubation.TheMix-n-Staindyeisnolongerreactiveattheendofthelabeling,sotheconjugateisreadyforstainingwithoutfurtherpurification.Afterlabeling,thedyeiscovalentlylinkedtotheantibodywithadegreeoflabelingofapproximately4-6dyemoleculesperantibodymolecule.Mix-n-Stainlabelingiscovalent,soMix-n-Stain-labeledantibodiescanbeusedformulticolorfluorescencestainingwithouttransferofdyesbetweenantibodies.Mix-n-Stainlabelingcantoleratesodiumazide,andlowlevelsofglycerol,Tris,andglycine.AmicrocentrifugeultrafiltrationvialisprovidedinthekittorapidlyremoveincompatIBLesmallmoleculeantibodystabilizersbeforelabeling.ThestandardMix-n-Stainlabelingprotocolcanbeperformedinthepresenceofuptofour-foldexcessofBSAorgelatintoIgG(byugamount).SimplychoosethekitsizethatcorrespondstotheamountofIgGyouwishtolabel.AmodifiedprotocolisprovidedforlabelingIgGinthepresenceofexcessstabilizerproteinorascitesfluid.Inthiscase,choosethekitsizethatcorrespondstothetotalamountofprotein(IgG+stabilizer,ortotalproteinamountinascitesfluid)intheantibodysampleyouwishtolabel.ThemodifiedprotocolalsocanbeusedtolabelamountsofIgGthatfallbelowthelowerrangeofthekitbyaddingstabilizerproteintotheIgGtobringthetotalproteinamountwithinthekitrange.Themodifiedprotocolisnotrecommendedforlabelingantibodiesincrudeantiserumorhybridomacellculturesupernatantduetothelowconcentrationofantibodyrelativetototalproteinintheseformats.Whenperformingdirectimmunofluorescencewithafluorescently-labeledantibody,youmayneedtouseahigherconcentrationofantibodytoachievesimilarstainingintensitycomparedtoindirectimmunofluorescencedetectionusingunlabeledprimarypluslabeledsecondaryantibody.Inourinternaltesting,indirectimmunofluorescencestainingresultsinabout3-foldsignalamplificationcomparedtodirectimmunofluorescencestaining.LabeledsecondaryantibodieswillstillbindtoprimaryantibodieslabeledusingMix-n-Stainkits,thereforeifmultipleprimaryantibodiesfromthesamespeciesaretobeusedformulticolorimmunofluorescencestaining,asecondaryantibodycannotbeusedtodistinguishanunlabeledprimaryantibodyfromaMix-n-Stainlabeledprimaryantibodyfromthesamespecies.Mix-n-Stainlabeledantibodiescanbeusedasatertiarystainingantibodyafterstandardimmunofluorescencestainingwithprimaryandsecondaryantibodies.AvivaalsooffersbiotinMix-n-Stainlabelingkits,forsecondarydetectionusingCF™dye-labeledstreptavidinorCF™dye-labeledmonoclonalmouseanti-biotin. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
