Application:FC, IFAdditional Information:Technical SummaryAbs/Em Maxima: 562/583 nmExtinction coefficient: 100,000Molecular weight: ~ 714Excitation laser line: 532 nm or 568 nmDirect replacement for: Alexa Fluor® 568, ATTO 565, Rhodamine Red:::Advantages:-Optimized for the 568 nm line of the Ar-Kr mixed-gas laser -Brighter and more photostable than Alexa Fluor 568:::| Component | 20-50 ug kit |
| Dye vial | (1) vial Component A |
| Mix-n-Stain reaction | (1) vial of 15 uL buffer, 10X Part # 99951-1 |
| Mix-n-Stain antibody | (1) vial of 150 uL storage buffer Part # 99952-1 |
| Ultrafiltration vial (MWCO=10K) | 1 each Part # 99956 |
:::Please visit www.avivasysbio.com to view our full selection of products featuring bright and photostable CF™ dyes, including secondary antibodies, streptavidin, anti-biotin, and anti-tag antibodies. Aviva also offers a variety of apoptosis and cell viability assays for flow cytometry analysis, including mitochondrial membrane potential dyes, fluorescent Annexin V conjugates, and NucView™488 Caspase-3 Substrate for live cells.CF dye, Mix-n-Stain, and modified Mix-n-Stain labeling technologies are covered by pending US and international patents. TWEEN is a registered trademark of Uniqema Americas LLC.Aviva products are high-quality reagents and materials intended for research purposes only. Some products are potentially hazardous chemicals - please read the Material Safety Data Sheet for additional information regarding handling potentially hazardous chemicals. Several of Aviva products and product applications are covered by U.S. and international patents and pending patents. Our products are not available for resale or other commercial uses without a specific agreement from Aviva Systems Biology Corporation. We welcome inquiries about licensing the use of our dyes, trademarks or technologies. Please submit inquiries by e-mail to info@avivasysbio.comReconstitution and Storage: -20CStability: Stable for at least 3 months from date of receipt when stored as recommended.Datasheets/Manuals:Printable datasheet/reference manual for Mix-n-Stain CF568 Antibody Labeling Kit,1x(20-50ug) labeling (OKBE00027) OKBE00027Description of Target:Mix-n-Stain™ antibody labeling kits contain everything you need to rapidly label an antibody with Aviva"s next-generation CF™ dyes or biotin. The labeling procedure comprises simple mixing of your antibody with the reaction buffer and optimally formulated dye provided, followed by a brief incubation. The Mix-n-Stain dye is no longer reactive at the end of the labeling, so the conjugate is ready for staining without further purification. After labeling, the dye is covalently linked to the antibody with a degree of labeling of approximately 4-6 dye molecules per antibody molecule. Mix-n-Stain labeling is covalent, so Mix-n-Stain-labeled antibodies can be used for multicolor fluorescence staining without transfer of dyes between antibodies.Mix-n-Stain labeling can tolerate sodium azide, and low levels of glycerol, Tris, and glycine. A microcentrifuge ultrafiltration vial is provided in the kit to rapidly remove incompatible small molecule antibody stabilizers before labeling.The standard Mix-n-Stain labeling protocol can be performed in the presence of up to four-fold excess of BSA or gelatin to IgG (by ug amount). Simply choose the kit size that corresponds to the amount of IgG you wish to label. A modified protocol is provided for labeling IgG in the presence of excess stabilizer protein or ascites fluid.In this case, choose the kit size that corresponds to the total amount of protein (IgG + stabilizer, or total protein amount in ascites fluid) in the antibody sample you wish to label. The modified protocol also can be used to label amounts of IgG that fall below the lower range of the kit by adding stabilizer protein to the IgG to bring the total protein amount within the kit range. The modified protocol is not recommended for labeling antibodies in crude antiserum or hybridoma cell culture supernatant due to the low concentration of antibody relative to total protein in these formats.When performing direct immunofluorescence with a fluorescently-labeled antibody, you may need to use a higher concentration of antibody to achieve similar staining intensity compared to indirect immunofluorescence detection using unlabeled primary plus labeled secondary antibody. In our internal testing, indirect immunofluorescence staining results in about 3-fold signal amplification compared to direct immunofluorescence staining.Labeled secondary antibodies will still bind to primary antibodies labeled using Mix-n-Stain kits, therefore if multiple primary antibodies from the same species are to be used for multicolor immunofluorescence staining, a secondary antibody cannot be used to distinguish an unlabeled primary antibody from a Mix-n-Stain labeled primary antibody from the same species. Mix-n-Stain labeled antibodies can be used as a tertiary staining antibody after standard immunofluorescence staining with primary and secondary antibodies. Aviva also offers biotin Mix-n-Stain labeling kits, for secondary detection using CF™ dye-labeled streptavidin or CF™ dye-labeled monoclonal mouse anti-biotin. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
