Predicted Species Reactivity:Human, Mouse, RatProduct Format:LiquidClonality:PolyclonalHost:RabbitApplication:IHC-P, WB, IPReconstitution and Storage:Store at 2-8C for up to 1 year. Avoid repeated freeze-thaw cycles.Replacement Item:This antibody may replace item sc-129376 from Santa Cruz Biotechnology.Immunogen:CDKN1B / p27 Kip1 antibody was raised against synthetic peptide from human CDKN1B / p27 Kip1.Purification:Affinity purifiedConcentration:0.2 mg/mlDatasheets/Manuals:Printable datasheet for OALA02664Specificity:p27Epitope:C-TerminusWarning:For research use only.Application Info:IHC - Paraffin (10 ug/ml), Western blot (1 - 2 ug/ml), Immunoprecipitation (300 - 500 ug/1E6 cells)Application Data:Immunohistochemistry: OALA02664 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02664 was determined to be 10 ug/ml.Storage Buffer:PBS, pH 7.4, 0.02% sodium azide.Gene Symbol:CDKN1BAlias Symbols:CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4NCBI Gene Id:1027Swissprot Id:P46527Protein Accession #:NP_004055.1Nucleotide Accession #:NM_004064Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express CDKN1B. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express CDKN1B. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
