Predicted Species Reactivity:Human, Monkey, Mouse, Rat, BovineProduct Format:LiquidClonality:PolyclonalIsotype:IgGHost:RabbitApplication:IHC-P, WBReconstitution and Storage:Short term 4C, long term aliquot and store at -20C, avoid freeze thaw cycles.Immunogen:KLF4 antibody was raised against synthetic peptide from human KLF4.Purification:Protein G purifiedConcentration:0.5 mg/mlDatasheets/Manuals:Printable datasheet for OALA02165Specificity:KLF4Epitope:aa1-50Warning:For research use only.Application Info:IHC - Paraffin (10 ug/ml), Western blot (1 - 3 ug/ml)Application Data:Immunohistochemistry: OALA02165 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for OALA02165 was determined to be 10 ug/ml.Storage Buffer:PBS, 0.05% BSA, 0.05% sodium azide.Gene Symbol:KLF4Alias Symbols:KLF4, EZF, GKLF, Kruppel-like factor 4 (gut), Hezf, Krueppel-like factor 4NCBI Gene Id:9314Swissprot Id:O43474Protein Accession #:NP_004226.3Nucleotide Accession #:NM_004235Tissue Tool:Find tissues and cell lines supported by DNA array analysis to express KLF4. RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express KLF4. 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物,这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌,哺乳动物细胞和昆虫细胞中表达,并作为整体裂解物提供。