Tested Species Reactivity:HumanPredicted Species Reactivity:Cow, Dog, Guinea Pig, Horse, Human, Mouse, Pig, Rabbit, Rat, Yeast, ZebrafishProduct Format:Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.Clonality:PolyclonalHost:RabbitApplication:IHC, WBAdditional Information:IHC Information: Paraffin embedded liver tissue, tested with an antibody dilution of 5 ug/ml.Reconstitution and Storage:For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.Replacement Item:This antibody may replace item sc-100783, HPA000704, HPA001290Immunogen:The immunogen is a synthetic peptide directed towards the middle region of human MTHFD1Purification:Affinity PurifiedPredicted Homology Based on Immunogen Sequence:Cow: 93%; Dog: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Yeast: 79%; Zebrafish: 79%Complete computational species homology data:Anti-MTHFD1 (ARP46147_P050)Peptide Sequence:Synthetic peptide located within the following region: CMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTMSTMPGConcentration:Batch dependent within range: 100 ul at 0.5 - 1 mg/mlBlocking Peptide:For anti-MTHFD1 (ARP46147_P050) antibody is Catalog # AAP46147 (Previous Catalog # AAPS17002)Datasheets/Manuals:Printable datasheet for anti-MTHFD1 (ARP46147_P050) antibodyTarget Reference:Palmieri,A., Ann. Hum. Genet. 72 (PT 3), 297-299 (2008)Gene Symbol:MTHFD1Official Gene Full Name:Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetaseAlias Symbols:MTHFC, MTHFDNCBI Gene Id:4522Protein Name:C-1-tetrahydrofolate synthase, cytoplasmicDescription of Target:MTHFD1 possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.Swissprot Id:P11586Protein Accession #:NP_005947Nucleotide Accession #:NM_005956Protein Size (# AA):935Molecular Weight:101kDaTissue Tool:Find tissues and cell lines supported by DNA array analysis to express MTHFD1. 
RNA Seq:Find tissues and cell lines supported by RNA-seq analysis to express MTHFD1. 
Protein Interactions:UBC; FUS; SUMO2; SUMO3; NEDD8; MDM2; RPA3; RPA2; RPA1; RNF2; RPS21; MAT2A; UBQLN1; FBXO6; UBD; ADRB2; CD81; VCAM1; HSP90AA1; HSP90AB1; PRKCDBP; SFXN1; TMEM165; IARS2; SARS2; SF3B3; FAF2; PPT2; SNRNP70; RPL32; MRPL23; ALDH18A1; PRPH; PC; ICT1; GAA; ACSL3; 我们在Aviva Systems Biology生产并提供含有重组蛋白或天然蛋白的裂解物，这些蛋白可在Western blot检测中用作阳性对照。重组蛋白在细菌，哺乳动物细胞和昆虫细胞中表达，并作为整体裂解物提供。
