| Predicted Species Reactivity | Human, Mouse |
|---|---|
| Product Format | Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | WB, IHC |
| Additional Information | Packaging : 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
| Reconstitution and Storage | For long term storage -20C is recommended. Stable at -20C for at least 1 year. |
| Immunogen | Peptide from the intracellular C-terminus region of human dopamine transporter (DAT), conjugated to keyhole limpet hemocyanin (KLH). |
| Purification | Prepared from rabbit serum by affinity purification using a SulfoLink column matrix to which the peptide immunogen was coupled. |
| Predicted Homology Based on Immunogen Sequence | Human; Mouse |
| Datasheets/Manuals | Printable datasheet for OAPC00104 |
| Specificity | Specific for the ~88k DAT protein in Western blots. |
| Application Info | The antibody has been tested in Western blots of SDS-solubilized human and mouse striatal samples and in IHC applications with monkey (Macaque) brain sections.Western blot of human caudate lysate showing specific immunolabeling of the ~88k DAT protein. |
| Target Reference | Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.Wilson JM, Kalasinsky KS, Levey AI, Bergeron C, Reiber G, Anthony RM, Schmunk GA, Shannak K, Haycock JW, Kish SJ (1996) Striatal dopamine nerve terminal markers in human, chronic methamphetamine users. Nat Med 2:699-703.Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286. |
| Gene Symbol | SLC6A3 |
|---|---|
| NCBI Gene Id | 6531 |
| Protein Name | Sodium-dependent dopamine transporter |
| Description of Target | The dopamine transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson"s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996). |
| Swissprot Id | Q01959 |
| Protein Accession # | NP_001035.1 |
| Molecular Weight | ~88kDa |
| Tissue Tool | Find tissues and cell lines supported by DNA array analysis to express SLC6A3.  |
| RNA Seq | Find tissues and cell lines supported by DNA array analysis to express SLC6A3.  |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
- IHC Tips & Tricks
- ICC Tips & Tricks
- ELISA Tips & Tricks
- WB/IB Tips & Tricks
- IP Tips & Tricks
See our General FAQ page.
What is the species homology for "Anti-Dopamine Transporter, C-Terminus (OAPC00104)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse".
How long will it take to receive "Anti-Dopamine Transporter, C-Terminus (OAPC00104)"?
This item is available "Domestic: within 1 week delivery | International: 1 week".
What buffer format is "Anti-Dopamine Transporter, C-Terminus (OAPC00104)" provided in?
This item is provided in "Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.".Additional format options may be available. For more information please contact info@avivasysbio.com.
What are other names for "Anti-Dopamine Transporter, C-Terminus (OAPC00104)"?
This target may also be called "" in publications.
What is the shipping cost for "Anti-Dopamine Transporter, C-Terminus (OAPC00104)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
What is the guarantee for "Anti-Dopamine Transporter, C-Terminus (OAPC00104)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
Can I get bulk pricing for "Anti-Dopamine Transporter, C-Terminus (OAPC00104)"?
You can get bulk pricing for this item by going here.
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "~88kDa".Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.
What protocols are available for "Anti-Dopamine Transporter, C-Terminus (OAPC00104)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
What are positive controls for "SLC6A3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What are negative controls for "SLC6A3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
What other proteins interact with "SLC6A3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
What biological processes are associated with "SLC6A3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
What cellular components are associated with "SLC6A3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
What protein functions are associated with "SLC6A3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
