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苏州工业园区生物纳米园A4#216
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商品描述
| CatalogNumber: 26903 |
| GeneSymbol: RAC1 |
| Description: Anti-ActiveRac1-GTPMouseMonoclonalAntibody |
| Background: SmallGTPasesareasuper-familyofcellularsignalingregulators.RacbelongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofRac,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs). |
| Immunogen: RecombinantfulllengthproteinofactiveRac1 |
| Applications: IP,IHC |
| RecommendedDilutions: 1µgfor1~2mgtotalcellularproteinsforIP; 1:100dilutionforIHC |
| Concentration: 1mg/ml |
| Host: mouse |
| Format: Liquid |
| Clonality: Monoclonal |
| Isotype: IgM |
| Purity: Purifiedfromascites |
| Storagebuffer: |
| Preservative: no |
| Constituents: PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol |
| SpeciesReactivity: Anti-activeRac1antibodyrecognizesactiveRac1fromvertebrates. |
| StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles |
DownloadDatasheet:  |
| Immunoprecipitation/Westernblot: |
| Racactivationassay.MEFcellsweretreatedwith(lane2)orwithout(lane1)PDGF.Celllysateswereincubatedwithananti-activeRacmonoclonalantibody(Cat#26903)(toppanel).ThprecipitatedactiveRacwasimmunoblottedwithananti-Racrabbitpolyclonalantibody(Cat#21003).ThebottompanelshowstheWesternblotwithanti-Racofthecelllysatesused(5%ofthatusedinthetoppanel). |
| Immunofluorescence: |
 | ImmunofluorescencefortheactiveRacusingAnti-ActiveRac1-GTPMouseMonoclonalAntibody[26903]showsRac-GTPimmunolabeling(green)incombinationwithcofilin(red)onbraintissuesections.Thetissuesectionswerefixedwith-20ºCmethanolor4%paraformaldehyde(fixationtime1hr)andstainedwithantibodyat1:1000in0.1MPhosphatebufferwith0.3%TritonX,and4%BSAfor24hatroomtemperature.Secondaryantibodieswereanti-mouseAlexaFluor488andanti-rabbitAlexaFluor594at1:1000. |
| RelatedProducts | |||||
| Catalog# | Name | Size | Price | DataSheet | |
| 80501 | RacActivationAssayKit | 20assays | $637 |  | |
| 26903 | ActiveRac1-GTPMonoclonalAntibody | 30μL | $495 | | |
| 26005 | Anti-Rac1MouseMonoclonalAntibody | 100μL | $239 | | |
| 21003 | Anti-Rac1RabbitPolyclonalAntibody | 100μL | $219 | | |
| 21027 | Anti-Rac2RabbitPolyclonalAntibody | 100μL | $219 | | |
Publications:| 1. Phosphorylationofα-tubulinbyproteinkinaseCstimulatesmicrotubuledynamicsinhumanbreastcellsCytoskeletonVolume71,Issue4,pages257–272,April2014 | 2. ImmunofluorescenceandConfocalMicroscopyofNeutrophilsNeutrophilMethodsandProtocolsMethodsinMolecularBIOLOGyVolume1124,2014,pp251-268 | 3. Alinkbetweenthenuclear-localizedsrGAP3andtheSWI/SNFchromatinremodelerBrg1MolecularandCellularNeuroscienceVolume60,May2014,Pages10–25 | 4. SHP2PhosphatasePromotesMastCellChemotaxistowardStemCellFactorviaEnhancingActivationoftheLyn/Vav/RacSignalingAxisTheJournalofImmunologyApril14,20141301155 | 5. ROBO1,atumorsuppressorandcriticalmolecularbarrierforlocalizedtumorcellstoacquireinvasivephenotype:StudyinAfrican-AmericanandCaucasianprostatecancermodelsInternationalJournalofCancerVolume135,Issue11,pages2493–2506,1December2014 | 6. TranscriptionfactorsNRF2andnf-kbarecoordinatedeffectorsoftheRHOfamily,GTPbindingproteinrac1duringinflammationJBiolChem.2014May30;289(22):15244-58 | 7. CanonicalandNon-canonicalG-proteinSignalingHelpsCoordinateActinDynamicstoPromoteMacrophagePhagocytosisofZymosanMolCellBiol.2014Nov15;34(22):4186-99 | 8. p53DownRegulatesPDGF-InducedFormationofCircularDorsalRufflesinRatAorticSmoothMuscleCellsPLoSOne.2014Sep23;9(9):e108257 | 9. LPA-mediatedmigrationofovariancancercellsinvolvestranslocalizationofGαi2toinvadopodiaandassociationwithSrcandβ-pixCancerLett.2015Jan28;356(2PtB):382-91 | 10. cAMPcontrolstherestorationofendothelialbarrierfunctionafterthrombin-inducedhyperpermeABIlityviaRac1activationPhysiologicalReportsPublished24October2014Vol.2no.e12175DOI:10.14814/phy2.12175 | 11. CisplatinatSub-toxicLevelsMediatesIntegrinSwitchinLungCancerCellsAnticancerResearchDecember2014vol.34no.127111-7117 | 12. Semaphorin-3FsuppressesthestemnessofcolorectalcancercellsbyinactivatingRac1Cancerletters.March1,2015Volume358,Issue1,Pages76–84. | 13. GeometrysensingthroughPOR1regulatesRac1activitycontrollingearlyosteoblastdifferentiationinresponsetonanofiberdiameterIntegrBiol(Camb).2014Dec24 | 14. InhibitionofRac1ActivityintheHippocampusImpairstheForgettingofContextualFearMemoryMolNeurobiol.2015Jan24 | |
