NewEast/Active Rab5-GTP [26911][30 µL]/26911

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¥9900.00
货号:26911
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品牌:NewEastBio
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商品描述

CatalogNumber:  26911
GeneSymbol:  RAB5
Description:  Anti-ActiveRab5MouseMonoclonalAntibody
Background:  Rab5isamemberofRab-familyGTPases.Rab5isarate-limitingcomponentofthemachineryregulatingthekineticsofmembranetrafficintheearlyendocyticpathway.
Immunogen:  RecombinantfulllengthproteinofactiveGao
Applications:  IP,IHC
RecommendedDilutions:  1µgfor~2mgtotalcellularproteinsforIP1:100dilutionforIHC
Concentration:  1mg/ml
Host:  mouse
Format:  Liquid
Clonality:  Monoclonal
Isotype:  IgG1
Purity:  Purifiedfromascites
Storagebuffer:
Preservative:  no
Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol
SpeciesReactivity:  Anti-activeRab5antibodyrecognizesactiveRab5proteinsofvertebrates.
StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles
DownloadDatasheet:  
Immunoprecipitation/Westernblot:  
Rab5activationassay.Purifiedfull-lengthRab5AproteinswereimmunoprecipitatedaftertreatedwithGDP(lane1)orGTPγS(lane2).Immunoprecipitationwasdonewiththeanti-activeRab5monoclonalantibody(Cat.No.26911).Immunoblotwaswithananti-Rab5polyclonalantibody(Cat.No.21151).
 
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Publications:
1.  VASPpromotesTGF-βactivationofhepaticstellatecellsbyregulatingRab11dependentplasmamembranetargetingofTGF-βreceptors    HepatologyVolume61,Issue1,pages361–374,January2015
2.  Rab5ActivityRegulatesGLUT4SortingintoInsulin-ResponsiveandNon-Insulin-ResponsiveEndosomalCompartments:APotentialMechanismforDevelopmentofInsulinResistance    Endocrinology.2014Sep;155(9):3315-28