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商品描述
| Catalog Number: 10147 |
| Product Name: Gαi2 Protein Q205L mutant |
| Synonyms: Guanine nucleotide binding protein, alphainhibiting activity polypeptide 2, GNAI2, Galphai2 |
| Source: Human, recombinant full length, His6-tag |
| Expression Host: E. coli |
| Molecular Weight: 40 kDa |
| Purity: >95% by SDS-PAGE |
| Introduction: Heterotrimeric G proteins arecritical cellular signal transducers. Gαi represents onesub-family of G proteins that could mediate the inhibition ofadenylyl cyclases. Other biochemical and physiological functions ofGαi proteins are being explored. |
| Amino Acid Sequence (1-355, Q205L) |
| MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECR QYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWAD HGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGG LRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF |
| Properties |
| Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl |
| Physical Appearance (form): White or clear |
| Concentration: 1 mg/mL |
| Storage: -80°C |
| Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water.Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solutionto protect the protein is recommended and using of non-ionicdetergents such as n-Dodecyl β-D-maltoside (DoDM) orpolyethylene detergents (e.g. C12E10) also help to stabilize theprotein. Avoid repeated freezing and thawing after reconstitution.The purity of His-tagged Gαi2 Q205L was determined by SDS-PAGEand Coomassie Brilliant Blue Staining. |
|
| References: 1. Blatt, C. et al., Proc. Nat. Acad. Sci. 85: 7642-7646, 1988.2. Bloch, D. B. et al., Am. J. Hum. Genet. 42: 884-888, 1988.3. Bray, P. et al., Proc. Nat. Acad. Sci. 84: 5115-5119, 1987.4. Itoh, H. et al., J. Biol. Chem. 263: 6656-6664, 1988.5. Lan, K.-L. et al., J. Biol. Chem. 273: 12794-12797, 1998.6. Neer, E. J. et al., Hum. Genet. 77: 259-262, 1987.7. Ogden, S. K. et al., Nature 456: 967-970, 2008. |
Datasheet:  |
| Publications: |
| 1. Identification of novel signalling roles and targets for Galpha and Gbetagamma downstream of the insulin-like growth factor 1 receptor in vascular smooth muscle cells Biochem J. 2013 Feb 15;450(1):209-19 |
| 2. CRH activation of different signaling pathways results in differential calcium signaling in human pregnant myometrium before and during labor J Clin Endocrinol Metab. 2012 Oct;97(10):E1851-61 |
| 3. CXCR4 activation defines a new subgroup of Sonic hedgehog-driven medulloblastoma Cancer Res. 2012 Jan 1;72(1):122-32 |
| 4. Regulation of estradiol and progesterone production by CRH-R1 and -R2 is through divergent signaling pathways in cultured human placental trophoblasts Endocrinology. 2012 Oct;153(10):4918-28 |
| 5. WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins Cell Signal. 2011 Mar;23(3):550-4 |
| 6. Heterotrimeric Galpha(i) proteins are regulated by lipopolysaccharide and are anti-inflammatory in endotoxemia and polymicrobial sepsis Biochim Biophys Acta. 2011 Mar;1813(3):466-72 |
| 7. Estrogen- and xenoestrogen-induced ERK signaling in pituitary tumor cells involves estrogen receptor-α interactions with G protein-αi and caveolin I SteroidsVolume 77, Issue 5, April 2012, Pages 424–432 |
| 8. Na/H exchanger regulatory factors control parathyroid hormone receptor signaling by facilitating differential activation of G(alpha) protein subunits J Biol Chem. 2010 Aug 27;285(35):26976-86 |
| 9. Beta-arrestin- but not G protein-mediated signaling by the "decoy" receptor CXCR7 Proc Natl Acad Sci U S A. 2010 Jan 12;107(2):628-32 |
| 10. Structural basis for activation of trimeric Gi proteins by multiple growth factor receptors via GIV/Girdin Mol Biol Cell. 2014 Nov 5;25(22):3654-71 |
| 11. Canonical and Non-canonical G-protein Signaling Helps Coordinate Actin Dynamics to Promote Macrophage Phagocytosis of Zymosan Mol Cell Biol. 2014 Nov 15;34(22):4186-99 |
| 12. Fatty Acid-binding Protein 5 (FABP5) Regulates Cognitive Function Both by Decreasing Anandamide Levels and by Activating the Nuclear Receptor Peroxisome Proliferator-activated Receptor β/δ (PPARβ/δ) in the BrainJ Biol Chem. 2014 May 2;289(18):12748-58 |
